- FEI Tecnai F30 G2 Twin Transmission Electron Microscope — Reserve F30
Missouri’s newest high-end TEM will be beneficial to those working in nanotechnology, materials, and life sciences. It is capable of performing high resolution and quantitative analyses for serious investigators.
- Hitachi S-4700 Field Emission Scanning Electron Microscope — Reserve Hitachi
Our cold field emission SEM is capable of high resolution SEM imaging of materials and biological samples.
- FEI Quanta 600F Environmental SEM — Reserve Quanta — Reserve Bruker EDS
Missouri’s variable pressure SEM is perfect for biological samples and elemental analysis. It is equipped with a Bruker Quantax 200 Silicon Drift Detector which provides extremely high throughput rates and improved light element detection capabilities.
- JEOL JEM-1400 Transmission Electron Microscope — Reserve JEOL
This high performance, high contrast TEM is great for viewing biological samples, nanoparticles, and polymers.
- FEI Scios DualBeam FIB SEM
The combination of focused ion beam (FIB) and scanning electron microscopy (SEM) techniques allows site-specific analysis and precise ablation of materials on the micro scale.
Sample Preparation Equipment
- Wohlwend High Pressure Freezer
Rapid cryoimmobilization of biological specimen has many advantages over traditional processing methods. The HPF Compact 02 sets a new standard in high pressure freezing for biological samples up to 0.2 mm thickness and 0.6 mm³ volume. Its robust, reliable and tested design offers excellent cryofixation quality with great reproducibility.
- E.A. Fischione Model 1070 Nanoclean Plasma Cleaner — Reserve Plasma Cleaner
With the ability to control ratios of 3 different gases (Argon, Oxygen and Hydrogen), flow rates and time, the Model 1070 Nanoclean Plasma cleaner is ideal for cleaning residual hydrocarbons off TEM holders and samples, facilitating better analytical spectroscopy and imaging, as well as making carbon films on TEM grid hydrophilic or selectively thinning the films. Nanoclean 1070 User Manual
- Pelco Easiglow Glow Discharge — Reserve Glow Discharger
The Pelco Easiglow was a generous gift from Endowed Chair Linda Randall and has allowed University of Missouri investigators the ability to pursue protein structural analysis via negative staining and cryo-EM. The Easiglow temporarily alters the charge on a carbon film – this charge can be selected by altering the composition of the gas molecules in the chamber of the unit.
- EMS 150TES Sputter Coater — Reserve Sputter Coater
The EMC utilizes most commonly a platinum target to sputter a thin conductive layer on specimens that are imaged under the high vacuum of a scanning electron microscope. Targets available include: Pt, Au, Cr, Ta, Ti, Ni. Please see our “Sputter Coater Quick Guide” for simple operation.
- Emitech K950 Carbon Evaporator — Reserve Carbon Evaporator
For specimens that need a conductive layer for high vacuum SEM imaging, utilizing this instrument a thin (15 nm) carbon layer can be applied without interfering with X-ray energy dispersive spectroscopy or identification of elemental composition via backscattered electrons.
- FEI Mark IV Vitrobot — Reserve Vitrobot
The Mark IV Vitrobot is an automated vitrification robot to form thin vitreous films of ice < 1 micron in width on holey carbon films. Parameters to vary include volume, humidity, temperature, blot force, blot time, buffer salt, buffer pH, sample concentration.
- Gatan Ultrasonic Disc Cutter (Model 601)
For preparation of 3mm disc to fit into a TEM holder.
- Gatan Dimpler (Model 656)
This instrument takes a 3mm disc created with the Model 601 and dimples the specimen to assist with creation of an electron transparent specimen.
- Gatan Precision Ion Polishing System (Model 691)
Using two argon ion sources, a 3 mm disc is successively abraded under vacuum to create an electron-transparent specimen.
- Buehler Four-Stage Grinding and Two-stage polishing table — Reserve Polishing table
- Buehler Low Speed Diamond Saw
- Pelco Biowave
During traditional biological sample preparations, water within specimens is replaced by resin via dehydration. These solution exchanges take considerable time. Microwaves have been employed to facilitate faster solution exchange and are utilized for both TEM and SEM biological sample preparation.
- Tousimis AutoSamdri 815
- Leica Ultracut UCT Microtomes & FCS cryo-attachment
- Leica EM UC7 Ultramicrotome
- Leica EM Automated Freeze Substitution Device
- Leica KMR3 Knifemaker
- Spin Coater
Spin coater with 100 programmable settings. Chucks (1″, 1/2″ and 3/16″ vacuum spin chucks) for substrate sizes from 10 mm – 200 mm, silicon wafers, PMMA & developer available.
Funding for EMC instrumentation has been supported by multiple sources including several grants from the National Science Foundation, National Institutes of Health, University of Missouri’s Program for Research Infrastructure and Matching Expenses (PRIME) and different departmental Research Incentive Funds (RIF).