Courses & Labs
The Electron Microscopy Core Facility provides one-on-one training for both SEM and TEM. Please contact a core staff to be trained. Furthermore, we host courses and laboratory sections, including:
– Veterinary Pathobiology 8452 “Cell and Molecular Electron Microscopy” (Tommi White)
Physics 7230/4230 “Electron Microscopy and Microanalysis” (Tommi White) – funding for this course is suspended (2018)
Biochemistry 9001 “Protein Structure Determination by Electron Microscopy” – funding for this course is suspended (2018)
Physics 8560 “Quantitative X-ray Energy Dispersive Spectroscopy and Advanced Imaging” – funding for this course is suspended (2012)
Information for Students
Veterinary Pathobiology 8452 “Cell and Molecular Electron Microscopy”
Participating students will be introduced to biological TEM and SEM with a special emphasis on sample preparative methods. All students will pursue individual projects and will be able to get some hands-on experience by imaging their own specimen utilizing our electron microscopes.
Biochemistry 9001 “Protein Structure Determination by Electron Microscopy”
This course will introduce and familiarize students with the steps required to obtain 3D information related to protein structure. Included is general TEM operation, introduction to negative staining and variables affecting results, introduction to image processing, 2D & 3D alignment, classification and averaging, plunge freezing vitreous protein samples, and collecting cryo-TEM data. The class culminates with students applying their new found knowledge to their proteins of interest with 2-3 weeks for individual projects. Students gain up to 15 hours of TEM time throughout the course.
Physics 7230/4230 “Electron Microscopy and Microanalysis”
The Electron Microscopy Core Facility’s premier course for understanding theory behind and operation of electron microscopes. Lectures contain theory behind imaging and microanalysis with both scanning and transmission electron microscopes, laboratories demonstrate theory by hands-on operation of scanning (5 labs) and transmission (3 labs) electron microscopes ulitizing various imaging modes (high resolution imaging, secondary & backscattered imaging, variable pressure SEM, energy filtered imaging, STEM) with focus on microanalysis including X-ray energy dispersive spectroscopy (EDS), electron energy loss spectroscopy (EELS), energy filtered imaging (EFTEM), high angle annular dark field scanning transmission electron microscopy (HAADF STEM). Students will propose, perform and present novel imaging and microanalysis related to their research.
Lab 1. Introduction to SEM
Lab 2. Secondary and Backscattered Electron Signals
Lab 3. High Resolution SEM
Lab 4. Low Vacuum Mode in Environmental SEM
Lab 5. X-ray Energy Dispersive Spectroscopy for SEM
Lab 6. Introduction to TEM; Direct Alignments and HRTEM imaging
Lab 7. Scanning Transmission Electron Microscopy and X-ray Energy Dispersive Spectroscopy
Lab 8. Energy Filtered Imaging and Spectroscopy
Physics 8560 “Quantitative X-ray Energy Dispersive Spectroscopy and Advanced Imaging”
This course covers the theory and methodology to quantitatively analyze materials using both energy-dispersive (EDS) and wavelength-dispersive (WDS) spectrometry. Other topics include chemical mapping and data extraction using image processing and analysis techniques.
“Nanoscale Imaging and Analysis” Workshop
Over 4 days, participants in the “Nanoscale Imaging and Analysis” workshop will receive in-depth theory related to transmission electron microscopy and related analytical components available on the state’s most powerful transmission electron microscope (FEI Tecnai F30 G2 Twin). Material covered includes components, alignment and operation, image formation, high resolution TEM (HRTEM), high angle annular dark field scanning TEM (HAADF STEM), X-ray energy dispersive spectroscopy (XEDS), electron energy loss spectroscopy (EELS) and energy filtered imaging (EFTEM). After each morning’s lecture (~2 hours), students are paired and guided through hands-on operation to demonstrate the morning lecture’s theory (~2 hours) for a total of > 6 hours. The final day is reserved for students to perform individual imaging and microanalysis on their own specimens.
Lecture 1: TEM Basics: Theory
Lecture 2: TEM Basics: Alignment and Operation
Lecture 3: Introduction to High Resolution Transmission Electron Microscopy
Lecture 4: Scanning Transmission Electron Microscopy and X-ray Energy Dispersive Spectroscopy
Lecture 5: Energy Filtered Imaging and Analysis
F30 Lab Manual